Composite
Part:BBa_K2269027
Designed by: Jose Alvaro Ballesteros Gonzalez Group: iGEM17_Valencia_UPV (2017-09-17)
Tnos:YFP:Assembly Reporter PB:Luc:Tnos
Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 819
Illegal XbaI site found at 506 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 819
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 819
Illegal BglII site found at 302
Illegal BglII site found at 542
Illegal BglII site found at 649 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 819
Illegal XbaI site found at 506 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 819
Illegal XbaI site found at 506 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |